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Derivatization of Sodium Hyaluronate as a Possible Tool for Increasing of the Stability of Model Artificial Synovial Fluid
Hrochová, Eliška ; Mravec, Filip (referee) ; Kalina, Michal (advisor)
This master thesis deals with the optimization of the procedure of modification of hyaluronic acid structure for the use in the artificial synovial liquids. Based on the literature research, the amino acid alanine was used for the modification of carboxylic group in the glucuronic acid. The main subject of study is the improvement of the stability and mechanical properties of synovial liquid. DLS microrheology, macrorheology, thermogravimetric analysis (TGA), multi-angle light scattering with flow-field flow fractionation (AF4-MALS) and infrared spectroscopy (FTIR) were used for characterization. The theoretical part of this theses submits review of the musculoskeletal system, role of hyaluronic acid in metabolism and summary of synovial liquid. The experimental part focuses on the measurement of the stability and mechanical properties of three artificial samples (first with no modification, second with modified hyaluronic acid and third with modified hyaluronic acid and chondroitin sulphate). These samples were compared with real horse synovial fluid and artificial viscosupplement Orthovisc®.
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Derivatization of Sodium Hyaluronate as a Possible Tool for Increasing of the Stability of Model Artificial Synovial Fluid
Hrochová, Eliška ; Mravec, Filip (referee) ; Kalina, Michal (advisor)
This master thesis deals with the optimization of the procedure of modification of hyaluronic acid structure for the use in the artificial synovial liquids. Based on the literature research, the amino acid alanine was used for the modification of carboxylic group in the glucuronic acid. The main subject of study is the improvement of the stability and mechanical properties of synovial liquid. DLS microrheology, macrorheology, thermogravimetric analysis (TGA), multi-angle light scattering with flow-field flow fractionation (AF4-MALS) and infrared spectroscopy (FTIR) were used for characterization. The theoretical part of this theses submits review of the musculoskeletal system, role of hyaluronic acid in metabolism and summary of synovial liquid. The experimental part focuses on the measurement of the stability and mechanical properties of three artificial samples (first with no modification, second with modified hyaluronic acid and third with modified hyaluronic acid and chondroitin sulphate). These samples were compared with real horse synovial fluid and artificial viscosupplement Orthovisc®.
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The Efficiency of Chondroitin suphate, Glucosamine and other supplements of joint nutrition to the articular and musculoskeletal system in the world literature.
Pokorná, Kateřina ; Vilikus, Zdeněk (advisor) ; Tuka, Vladimír (referee)
Title The efficiency of Chondroitin sulphate, Glucosamine and other supplements of joint nutrition to the articular and musculoskeletal system in the world literature The aim of the work The aim of thesis is to assess the efficacy of glucosamine, chondroitin sulphate, hyaluronic acid and diacerein to the structure of joints with joint disease, especially osteoarthritis, according to world literature. Methodology Bachelor thesis is conceived as review of the world literature on the topic. The main method of the work was compilation meta-analysis based on an analysis of numerous quality publications and world scientific literature focused on the topic of bachelor thesis. Electronic databases EBSCO and PubMed and Internet search engine Google Scholar were used as resources for search. Results It found several high-quality studies concerning the influence of drugs of SYSADOA group on the structure of joints. Generally, drugs of this group may have some influence not only to symptoms accompanying osteoarthritis, but also the structure of joint tissues. However, some studies refute this claim. Therefore, it is necessary perform further extensive studies. The correlation between the stage of disease of the patient and the effectiveness of the individual active substances was found. Patients with moderate...
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